BibTeX:

@article{IJIRSTV2I9045,
     title={In Vitro Tissue Culture from Nodal Segments of Wild Traits of Withania somnifera L},
     author={Naveen Gaurav, A. P. Singh , Aviral Maithani , Hira Singh Gariya and Bhoora},
     journal={International Journal for Innovative Research in Science & Technology},
     volume={2},
     number={9},
     pages={78--81},
     year={},
     url={http://www.ijirst.org/articles/IJIRSTV2I9045.pdf},
     publisher={IJIRST (International Journal for Innovative Research in Science & Technology)},
}

Ashwagandha (Withania somnifera) is a one of the most important and reputed Indian traditional medicinal plant. Multiple shoot in vitro culture of Ashwagandha plants in two nutrient basal medium (i.e.in MS and B5 basal medium) were experimental established by using nodal excised small segments as explants. The hormonal combinations and mixture of BAP, 2,4-D and NAA not only enhanced there in vitro morphogenetic response but also differentially increases the level of bio-generation or biological production of withanolide A through in vitro shoots formation in two basal nutrient medium. Withanolide-A, that was normally present in high quantity and high proportion in the aerial region and parts of Ashwagandha (explants source) which is propagated and grown in field and now it is proof that their concentration regularly increased in the in vitro shoot or stem cultures by nodal excised segments of the Withania somnifera plant. The productivity and isolation of withanolide-A in the in vitro cultures varied and fluctuates between 0.014 - 0.14 mg/gram of fresh weight of Withania plant with the alternatively change in the growth hormone composition and concentration of the nutrient culture basal media as well as its genotype used as important source of the excised explants.

Ashwagandha, bio-generation, withanolide-A, in vitro shoots, hormonal combinations